Human Gamma Globulin Studies on Serum Proteins : Immunoelectrophoretic
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منابع مشابه
Sites of Formation of Immune Globulins and of a Component of C'3
The development of a new method for the determination of the sites of serum protein formation has been described. The method involves the incorporation of C(14)-labeled amino acids by tissues cultured in vitro, and subsequent autoradiography of immunoelectrophoretic patterns prepared from a mixture of culture fluids and carrier serum with an antiserum against the carrier serum. This technique h...
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Normal human urine has been shown to contain up to 150 mg. of protein in a 24 hour aliquot. Electrophoretic analyses of these proteins by a number of observers (1-3) have demonstrated the presence of components corresponding to each of the five major fractions present in serum. In general, however, the resolution of these peaks has been poor, and significant amounts of protein migrating more ra...
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The rheumatoid factor-like substance (RFLS) induced by hyperimmunization of rabbits with bacteria was consistently and exclusively associated with the macroglobulin fraction of the sera studied. The RFLS is separable from the bulk of serum proteins by zone centrifugation, DEAE cellulose chromatography, and gel filtration. Partially purified preparations of the RFLS were studied by immunoelectro...
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Dardas, T. J. (Michigan State University, East Lansing), and V. H. Mallmann. Electrophoretic and immunoelectrophoretic studies of sera from normal, tuberculous, and noninfected tuberculin-sensitive guinea pigs. J. Bacteriol. 92:76-81. 1966.-Normal guinea pig serum was separated into seven fractions by electrophoresis on cellulose acetate membranes. Thirty antigens were found by immunoelectropho...
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The fractional rates of catabolism of isotopically labeled mouse, human, bovine, and guinea pig gamma-globulins and human serum albumin were determined in mice and in guinea pigs whose serum gamma-globulin and serum albumin levels were elevated by immunization or by injections of exogenous serum proteins. These serum proteins were also followed in mice with different serum gamma-globulin levels...
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